Fig. 5

Comparison between H₂O₂-induced and mia40-4int-dependent changes in thiol oxidation a Left: comparison of difference in average % oxidation values for 1,785 unique cysteine-containing peptides quantified in both datasets. Lines indicate difference in average % oxidation ≥ 7. Right: zoom-in of the region with an average increase in oxidation of at least + 7% in mia40-4int and upon H₂O₂ treatment. Peptides are labelled with the respective protein names and the position(s) of cysteine residue(s) in the sequence. Unique cysteine-containing peptides that show an increase in thiol oxidation of at least + 7% with a P-value < 0.05 in mia40-4int (n = 4) and/or upon H₂O₂ treatment (n = 3) are highlighted as indicated. Cysteine-containing peptides highlighted in blue in the zoom-in also exhibited increased thiol oxidation in mia40-4int (≥ 7%) but with P-values > 0.05 (n = 4). b Functional protein association network of proteins which exhibited increased oxidation (≥ 7%) in mia40-4int and in WT yeast upon H₂O₂ treatment using STRING⁶³ (version 10.0). Proteins highlighted in red showed a significant increase in thiol oxidation in mia40-4int (P-value < 0.05, n = 4), as well as upon H₂O₂ treatment (P-value < 0.05, n = 3). Proteins highlighted in blue exhibited a significant increase in thiol oxidation upon H₂O₂ treatment (≥ 7%, P-value < 0.05, n = 3). Thiol oxidation of these proteins was also increased in mia40-4int (≥ 7%) with P-values > 0.05 (n = 4). c Comparison of the number of proteins annotated with the GO term ‘ribonucleoprotein complex’ with at least one unique cysteine-containing peptide with an increase in thiol oxidation of at least + 7% (P-value < 0.05) upon H₂O₂ treatment (n = 3) and/or in mia40-4int (n = 4)