Table 2 Transduction efficiency of zygotes from different strains of mice with rAAV6-EGFP

From: Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses

Genetic background

Treatment

Number of treated zygotes

Number of survivingb embryos (%)

Number of EGFP-positive embryos (%)

C57BL/6J

rAAV6-EGFPa

30

30 (100)

30 (100)

no rAAV

8

5 (63)

0 (0)

FVB/N

rAAV6-EGFP

24

16 (67)

16 (100)

no rAAV

12

11 (92)

0 (0)

CD-1

rAAV6-EGFP

9

9 (100)

9 (100)

no rAAV

8

7 (88)

0 (0)

  1. a Experimental embryos were exposed to viral particles for 5–6 h
  2. b Embryos that developed to compacted morula or blastocyst stage after 3 days in culture