Table 3 Ex vivo Cre recombination after transduction of R26mTmG zygotes with rAAV6-Cre

From: Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses

Number of treated zygotes

Time of analysis

Number of surviving embryosa or pups (%)

Number of EGFP-positive embryos or pups (%)

Number of mosaic embryos or pups (%)

40

E3.5

38 (95)

32 (84)

5 (16)

74

P2b

38 (51)

37 (97)

15 (37)

  1. a Embryos that developed to compacted morula or blastocysts after 3 days in culture
  2. b Embryos were cultured overnight, transferred into pseudopregnant females and analyzed at postnatal day 2
Back to article page