Table 2 HPLC conditions for analysis of digested and undigested synthetic RNA oligonucleotides
From: Detecting RNA base methylations in single cells by in situ hybridization
Column: Waters XSelect HSS T3, 100 Å, 3.5 µm, 3.0 × 150 mm | |||
|---|---|---|---|
Column temperature: 30 °C | |||
Flow rate: 0.7 mL min−1 | |||
Injection volume: 8 μL | |||
Step time / min | Mobile phase composition / % | ||
A (water) | B (acetonitrile) | C (0.2 M NH4OAc, pH 6, 25% acetonitrile) | |
0 | 89 | 1 | 10 |
2.5 | 89 | 1 | 10 |
5.5 | 80 | 10 | 10 |
10 | 40 | 50 | 10 |
10.1 | 10 | 80 | 10 |
11 | 10 | 80 | 10 |
11.1 | 89 | 1 | 10 |
20 | 89 | 1 | 10 |
