Table 3 HPLC conditions for analysis of digested ribosomal RNA
From: Detecting RNA base methylations in single cells by in situ hybridization
Column: Waters XSelect HSS T3, 100 Å, 3.5 µm, 3.0 × 150 mm | |||
|---|---|---|---|
Column temperature: 30 °C | |||
Flow rate: 0.7 mL min−1 | |||
Injection volume: 8 μL | |||
Step time / min | Mobile phase composition / % | ||
A (water) | B (acetonitrile) | C(0.2 M NH4OAc, pH 6, 2.5% acetonitrile) | |
0 | 90 | 0 | 10 |
3.6 | 90 | 0 | 10 |
6.0 | 88 | 2 | 10 |
7.5 | 85 | 5 | 10 |
13.5 | 70 | 20 | 10 |
24 | 55 | 35 | 10 |
27.5 | 10 | 80 | 10 |
28.8 | 10 | 80 | 10 |
29.0 | 90 | 0 | 10 |
38.0 | 90 | 0 | 10 |
