Fig. 1

Conditional knockout and developmental expression of NHE9. a Diagram shows the 12 predicted transmembrane domains (TMDs) of NHE9 and the location of two loxP sites (red arrowheads) between TMD3 and TMD4. Asterisks indicate the location of ASD-associated mutations. b Quantitative RT-PCR demonstrates the reduction in brain NHE9 mRNA from, respectively, heterozygous and conditional knockout (cKO) animals relative to WT and those with unrecombined alleles (flox/flox). Bars indicate the mean and error bars s.e.m. n = 2–4 animals/genotype. c Time course of brain NHE9 expression (by quantitative RT-PCR) from embryo to adulthood in wild-type C57Bl/6 mice. The data indicate mean ± s.e.m. n = 3 mice/time point