Fig. 1
From: Pharmacogenetic stimulation of neuronal activity increases myelination in an axon-specific manner
DREADD-mediated neuronal stimulation results in increased cFos expression. a–c PBCag-GFP (GFP control) or a 1:1 combination of PBCag-GFP and PBCag-hM3Dq (hM3Dq/GFP) were electroporated unilaterally into the right cerebral neuroepithelium of CD1 mice at E15.5 (a), resulting in a highly stereotypical and reproducible expression pattern in layer 2/3 S1 pyramidal neurons (b) and their axonal projections in the corpus callosum at P20 (c). d Structure of the PiggyBac plasmids encoding hM3Dq and Gfp that were used. e Representative images showing cFos expression in the S1 cortex of GFP control (top panel) and hM3Dq/GFP (bottom panel) mice following a week of CNO administration (P19–26). f Plasmid electroporation efficiency was similar between hM3Dq/GFP and GFP control mice. g The normalized absolute intensity of cFos immunostaining was significantly increased in hM3Dq/GFP electroporated neurons compared to that of GFP controls or non-electroporated neurons following a week of CNO-mediated stimulation (AU, arbitrary units). h Graph depicting the percentage change in cFos normalized absolute intensity from the average GFP− cell intensity within samples. Welch’s corrected unpaired two-tailed t-test: *P < 0.05, ***P < 0.001; n = 6 mice/group,  ± s.e.m. Scale bars = 500 µm (b), 200 µm (c, e), 10 µm (insets, e). See also Supplementary Fig. 1
