Fig. 2
From: Pharmacogenetic stimulation of neuronal activity increases myelination in an axon-specific manner
Activity stimulation enhances oligodendrogenesis in juvenile mice. a Experimental timeline illustrating onset of CNO/EdU administration in juvenile mice that had been electroporated with plasmids containing either hM3Dq and Gfp (hM3Dq) or Gfp only (Control) in utero at E15.5. b Coronal sections through the corpus callosum at P19+7 were immunolabeled with OPC (PDGFRa) and mature oligodendrocyte (ASPA) markers and the number of proliferating (EdU+) OPCs (open arrowheads, b’) and newly differentiated oligodendrocytes (filled arrowheads, b”) was quantified. c–f Activity stimulation resulted in increased levels of progenitor cell proliferation (c, d), as well as enhanced differentiation and oligodendrogliogenesis (e, f). Welch’s corrected unpaired two-tailed t-test: **P < 0.01, *P < 0.05; n = 4 Control mice (GFP only), n = 5 hM3Dq/GFP mice, ± s.e.m. Scale bars = 200 µm (b), 20 µm (b’, b”). See also Supplementary Figs. 2, 3
