Fig. 3

Activity stimulation in adult mice results in increased oligodendrogenesis. a Experimental timelime indicating onset of CNO/EdU administration in adult mice that had been electroporated with plasmids containing either hM3Dq and Gfp (hM3Dq) or Gfp only (Control) in utero at E15.5. b Coronal sections through the corpus callosum at P60+14 were immunolabeled with OPC (PDGFRa) and mature oligodendrocyte (ASPA) markers and the number of proliferating (EdU⁺) OPCs (open arrowheads, b’) and newly differentiated oligodendrocytes (filled arrowheads, b”) was quantified. c–f Activity stimulation resulted in increased levels of OPC proliferation and cell cycle entry (c, d), which in turn resulted in increased EdU⁺ incorporation in adult ASPA⁺ cells (f), although the difference in overall number of mature oligodendrocytes was not significant between the groups (e). Welch’s corrected unpaired two-tailed t-test: *P < 0.05; n = 4 mice/group,  ± s.e.m. Scale bars = 200 µm (b), 20 µm (b’, b”). See also Supplementary Fig. 4