Fig. 3 | Nature Communications

Fig. 3

From: Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis

Fig. 3

Chi3l1 KO CD4 T cells are prone to Th1 differentiation. a, b MACS-sorted WT and Chi3l1 KO naive CD4 T cells were differentiated into Th1, Th2, and Th17 cells under specific cytokine-skewing condition, and Intracellular cytokine staining performed to analyze lineage-specific cytokine expression. c IFNγ, TNFα, IL-4, and IL-17 production in culture supernatants were measured by ELISA. d Lineage specific mRNA levels were analyzed by quantitative RT-PCR. Each gene expression level was normalized to β-actin, and represented as relative expression to WT. Data are mean ± SD of eight sets of independent experiments (n = 16). n.s., not significant; *p < 0.05, **p < 0.01 (two-tailed Student’s t-test)

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