Fig. 4
Increased Th1 and CTL response in Chi3l1 KO T cells are IFNγ dependent. a Phosphorylation of STAT1, STAT4, STAT6 were analyzed by Western blotting. Relative densitometric analysis of Western blotting normalized to total STAT. b WT and Chi3l1 KO naive CD4 T cells were differentiated into Th1 cells with the indicated concentrations of IFNγ neutralizing antibodies and assessed for IFNγ and IL-4 expression by intracellular cytokine staining. c Proportion of IFNγ producing cells were represented by relative % of IFNγ to WT Th1. d FACS-sorted WT and Chi3l1 KO naïve CD4 T cells were treated with IFNγ and pSTAT1 level was analyzed by Western blotting. Densitometric values of band intensity was calculated by normalization to the value of β-actin. e Intracellular level of IFNγ and Granzyme B in CD8 T cells. f Proportion of IFNγ producing cells were represented as relative % of IFNγ to WT CD8 T cells without anti-IFNγ neutralizing antibody. g Proportion of Granzyme B expressing cells were represented as relative % of Granzyme B to WT CD8 T cells without anti-IFNγ neutralizing antibody. Data are mean ± SD of three sets of independent experiments (n = 6). n.s., not significant; *p < 0.05, ***p < 0.001
