Fig. 5
RNA transcriptomes of Chi3l1 KO T cells. a 100-bp pair-ended RNA-sequencing in naïve, Th1-skewed WT, and Chi3l1 KO CD4 T cells. The number of up-regulated genes were indicated as red, and down-regulated genes were indicated as green in each comparative analysis. b Heatmap analysis of genes of interest. c Scatterplot indicates either of over than two-fold up-regulated and down-regulated genes in WT Th1 versus Chi3l1 KO Th1 comparison. d Expression of genes of interest related to cytotoxicity and the IFNγ signaling pathway in Th1 cells were confirmed by quantitative RT-PCR. Each gene expression level was normalized to β-actin, and represented as relative expression to WT. e Quantitative RT-PCR was performed in activated CD8 T cells. Each gene expression level was normalized to β-actin, and represented as relative to WT. Data are mean ± SD of ten sets of independent experiments (n = 20). n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001 (two-tailed Student’s t-test)
