Fig. 7

Chi3l1 deletion in T cell inhibits pulmonary metastasis with increase of Th1 and CTL. a Genotyping PCR to confirm CD4 specific deletion of Chi3l1 in the mice. b Representative lung image from B16F10 melanoma injected WT and CD4-Chi3l1 KO mice. Scale bar, 2 mm c Number of pleural colonies in each lung was counted. Data are mean ± SEM of three sets of independent experiments and each dot in graphs represent an individual mouse. d Histology of lung sections by H&E staining. Scale bar, 200 m (e, f) IFNγ and/or TNFα producing CD4 T cells and CD8 T cells in the lung was analyzed by intracellular cytokine staining. % of IFNγ⁺, % of TNFα⁺, and % of TNFα⁺IFNγ⁺ was represented as scattered graph. g, h IFNγ and/or TNFα producing NK cells, and non-lymphocytic populations (NK1.1^-CD4^-CD8^-) in the lung was analyzed by intracellular cytokine staining. % of IFNγ⁺, % of TNFα⁺, and % of TNFα⁺IFNγ⁺ was represented as scattered graph. i mRNA expression of genes related to cytotoxicity and Th1 effector functions was analyzed by quantitative RT-PCR. Each gene expression level was normalized to β-actin. Data are mean ± SD of three sets of independent experiments and each dot in graphs represent an individual mouse. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001 (two-tailed Student’s t-test)