Fig. 1
From: Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins
Design and workflow of MGBA. Each glycan was conjugated to one region specific Luminex bead, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. After blocking the beads with 1% BSA in PBS (w/v), the beads were probed with biotinylated lectins, anti-glycan antibodies and glycan-binding proteins. After washing, the bound lectins, anti-glycan antibodies and glycan-binding proteins were detected using phycoerythrin labelled streptavidin (SAPE). The unbound SAPE was removed by washing, and beads were resuspended in wash buffer. The beads were read in FLEXMAP 3D, as per settings defined in “Methods” secrion. The median fluorescence intensity data is then presented as mean+standard deviation of two replicates; each experiment was repeated three times
