Fig. 5
From: Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins
Natural anti-glycan IgM antibodies in 613 human serum samples measured on MGBA. a Dot plots of anti-IgM antibody levels (MFI) for each glycan in 613 individuals. Each dot represents one individual and mean for each glycan is represented by the red line. Background-subtracted net MFI data (MFI×1000) on y-axis is expanded for 0–20,000 range to improve visualization. b Heatmap of anti-IgM antibody levels in 613 samples. The data are Log 2 transformed. Each row represents an individual sample and each column represents an individual glycan. IgM levels are represented by the color codes as indicated by the color bar. Serum IgM was used to cluster glycans (Cluster 1–5) as well as subjects (Cluster a–d). Cluster A, B, C and D contain 221 (36%), 54 (8.8%), 325 (53.1%), and 13 (2.1%) subjects, respectively. The sample clusters are not associated with demographic or clinical variables such as collection date, gender and age of the subjects, and batch of sample analyses
