Fig. 6
Rbfox1 colocalizes with P-bodies and stress granules in the cytoplasm. a In the germline, Rbfox1 co-stains with the P-body marker Pacman. Note that all Pacman-positive granules are also Rbfox1-positive, but not all Rbfox1 foci stain with Pacman. b, c Upon miR-980 loss or starvation, which leads to elevated Rbfox1 levels, the appearance of P-bodies increases. d Upon temperature stress, Rbfox1 associates with the stress granules marked with anti-Hsp70/Hsc70 in the germarium and later stage egg chambers (g. and st.6 shown in lower panels). e Stress granules are undetectable in non-stressed ovaries. f Schematic drawing depicts a summary of subcellular localization of Rbfox1 protein. Rbfox1 is associated with the major membraneless organelles that regulate RNA metabolism, such as nucleoli and Cajal bodies in the nucleus and P-bodies, stress granules and amyloid-like fibers in the cytoplasm. Rbfox1 (green); DAPI (nuclei, blue); Pacman (P-bodies, red); Hsp70/Hsc70 (stress granules, red). Egg chambers are shown in a–e. Developmental stages (st.) of egg chambers are marked, germarium (g.). Images in a–e are single Z-sections. Scale bars 5 µm
