Fig. 4

CNT tape is feasible for light microscopy. a Emission spectrum, b Absorption spectrum, c Transmission spectrum, d Total light transmission, and e Haze of the CNT, uncoated Kapton and cc-Kapton tape. f, g GABA post-embedding immunoreaction with 15 nm colloidal gold particles on serial sections of rat striatum processed with TO on the CNT tape imaged with BSD at 5 keV, 3.2 µs dwell time. GABAergic synapse (white arrow) and non-GABAergic synapse (arrow). Scale, 0.5 µm. h, i Enlarged image of GABAergic synaptic contact indicated by a white arrow in f and g. Scale, 0.2 µm. j GABA post-embedding immunofluorescent reaction on serial thin sections of rat cortex processed with TOLA on the CNT tape. Scale, 80 µm. k Enlarged image of rectangle in j. GABA immunoreactive neurons (arrows) and basket like boutons (asterisks). Scale, 20 µm