Fig. 5

Effect of PMI on splicing. a Distribution of the number of exons with significant differential inclusion across the different tissues; inset: number of exons with differential inclusion occurring in multiple tissues. b, c Examples of two exons with PMI-correlated inclusion levels. SRSF3 and SF1 encode pre-mRNA splicing factors that form part of the spliceosome. d Splicing Entropy for the APBB1IP gene depending PMI in the lung tissue. e APBB1IP gene has three isoforms. The heatmap shows the proportion of each isoform in the Lung samples sorted by PMI (1 indicates that only one isoform contributes to the expression of the gene and 0 that the isoform is not expressed) PMI of the samples range from 49 to 1558 minand are represented in the green to red gradient scale bar. Exon structure (not to scale) for the three isoforms is represented below. This figure depicts how the expression of the longer transcript in this gene becomes less dominant as PMI increases, and, as a consequence, the abundance of the different isoforms tends to converge