Fig. 7
From: Mutations in CFAP43 and CFAP44 cause male infertility and flagellum defects in Trypanosoma and human
Orthologs of T. brucei TbCFAP44 and TbCFAP43 are flagellar proteins apposed to DMTs 5–6. a–f STED confocal microscopy, deconvolution, and 3D reconstruction views of the co-labeling, on permeabilized whole cells, of tubulin (green), PFR2 (gray), and 10TY1TbCFAP44 (a–c) and 10TY1TbCFAP43 (d–f) showing that TbCFAP44 and TbCFAP43 are facing the PFR and are closely associated with the tubulin, which likely corresponds to DMTs 5–6. Scale bars=0.5 μm. g–j Electron immunogold labeling of cytoskeleton-extracted cells expressing TbCFAP44myc (g) and TbCFAP43myc (h). Controls with no primary antibody (i) and anti-PFR2 (j). Gold beads size was either 6 nm (g, h) or 10 nm (i, j). Scale bars=200 nm in g, h; 100 nm in i, j. Insets are enlargement of images of flagella taken from the main panels and scale bars=10 nm
