Fig. 6
From: An evolutionary NS1 mutation enhances Zika virus evasion of host interferon induction
The role of NS1 mutation in evasion of IFN-β production in primary dendritic cells. BMDCs from C57BL/6J mice or Irf3−/− mice were infected with FSS13025 WT, FSS13025 A188V (a), PRVABC-59 WT, or PRVABC-59/V188A (b) at an MOI of 0.2. Cells were harvested and total intracellular RNA were isolated at days 1, 2, and 4 p.i., followed by qRT-PCR quantification of IFN-β mRNA (a, b) and intracellular viral RNA (c, d) as described in Fig. 5. e Extracellular viral RNA from C57BL/6J BMDC cells in (a and b) were measured by qRT-PCR. Data are presented as means ± SD from two independent experiments (with at least triplicates for each experiment). Statistical significance is presented as *P < 0.05, **P < 0.01, or no significance (n.s.) using unpaired Student’s t-test
