Fig. 4
From: The IAP family member BRUCE regulates autophagosome–lysosome fusion
BRUCE localizes to lysosomes but has no effect on lysosomal functions. a Immunofluorescent staining of endogenous LAMP2 and BRUCE in Atg5+/+ and Atg5−/− MEFs. Atg5+/+ and Atg5−/− MEFs in FM, starved for 4 h with or without Bafilomycin A1 (100 nM) were fixed and stained with anti- LAMP2 and anti- BRUCE antibodies. Fluorescent intensity of LAMP2 and BRUCE signals across 6 µm regions marked with arrows is shown in line plots. b Mean LysoTracker Red (LTR) signal in Bruce+/+ and Bruce−/− MEFs cells analyzed by flow cytometry. MEFs grown in regular and 6 h-starved conditions with or without Bafilomycin A1. Data are presented as mean±SD from three biological replicates (NS, not significant). Representative data are shown from three independent experiments
