Fig. 1
From: Bismuth antimicrobial drugs serve as broad-spectrum metallo-β-lactamase inhibitors
CBS inhibits the in vitro activity of MBLs. a Basic dimeric unit of CBS. b, c Representative heat plots of microdilution checkerboard assay for the combination of MER and CBS against NDM-HK (b) and NDM-HK PCV (c). d Time kill curves for MER and CBS monotherapy and combination therapy against NDM-HK during 24 h incubation. The concentrations of MER and CBS are 24 μg mL-1 and 64 μg mL-1, respectively. e Inhibition profiles for MBLs by CBS with IC50 values of 2.81, 3.54, and 0.70 µM for NDM-1, VIM-2, and IMP-4, respectively. Mean value of three replicates are shown and error bars indicate the standard deviation (SD). f Isobolograms of the combination of MER and CBS against different MBL-positive bacterial strains. The gray line indicates ideal isobole, where drugs act additively and independently. Data overlapping with this line indicate additive effects. Data points below this line indicate synergism
