Fig. 3
From: A NIN-LIKE PROTEIN mediates nitrate-induced control of root nodule symbiosis in Lotus japonicus
Structure of the NRSYM1 gene. a Exon–intron structure of the NRSYM1 gene. Black boxes indicate exons. Arrowheads indicate locations of the nrsym1 mutations. b Structure of the deduced NRSYM1 protein. An N-terminal conserved region is indicated by the gray box. C-terminal regions containing an RWP-RK DNA-binding domain (RWP-RK) and a conserved domain (PB1) are shown as black boxes. Arrowheads indicate locations of the nrsym1 mutations. c Complementation of the nrsym1 nodulation phenotype. Representative transgenic hairy roots of L. japonicus carrying a control empty vector or a 7.5-kb genomic fragment encompassing the entire NRSYM1 locus. Transgenic roots were identified by GFP fluorescence. The number of nodules was counted in the transgenic roots of nrsym1-1 mutants containing either an empty vector or a genomic fragment of NRSYM1 in the presence of 0 or 10 mM KNO3 at 21 dai (n = 8–10 plants). *P < 0.05 by Student’s t-test. ns, not significant. Degrees of freedom are shown above the t-values. Error bars indicate SEM. Scale bars: 1 mm
