Fig. 7
From: A NIN-LIKE PROTEIN mediates nitrate-induced control of root nodule symbiosis in Lotus japonicus
Effects of loss-of-function mutations in CLE-RS1/2 on nodulation. a The number of nodules and (b) maximum nodule diameter (mm) in WT, the cle-rs1 mutants (T2), the cle-rs2 mutants (T2), and the cle-rs1 -rs2 double mutants grown in the presence of 0 or 10 mM KNO3 (n = 7–14 plants). The cle-rs1 -rs2 double mutants were obtained by crossing the single mutants. The cle-rs1 and -rs2 mutants were obtained by the CRISPR/Cas9 genome-editing system (Supplementary Fig. 9a). Error bars indicate SEM. Columns with the same lower-case letter indicate no significant difference (Tukey’s test, P < 0.05; a). *P < 0.05 by Student’s t-test. Degrees of freedom are shown above the t-values (b). Scale bars: 1 mm
