Fig. 1

Characterization of naturally occurring DNA methylation variation region 19 (NMR19) in Col-0 and C24. a Integrated Genome Browser (IGB) snapshot (left) showing methylation variation between Col-0, C24 and its F1 hybrids (Col-0XC24/F1 and C24XCol-0/F1) generated from BS-seq data. Black bar below the IGB snapshot indicates the site of restriction enzyme used for DNA methylation-sensitive PCR (Chop-PCR). Gel picture (right) depicts the validation of NMR19 methylation levels by using Chop-PCR. b Chop-PCR analysis of methylation patterns of NMR19 in F2 population from reciprocal crosses between Col-0 and C24