Fig. 5
Methylation of NMR19-4 is negatively associated with leaf senescence. a The flanking gene structure of NMR19. black box: exon; black line: intron. Tip of the black box in gene structure depicts direction of transcription. b The expression of PPH was repressed in accessions with NMR19-4m as compared to the accessions with NMR19-4u or deleted NMR19. p-value was calculated by a two-tailed t-test, the same below. Error bars are defined as s.e.m. (n = 3). The expression and Chl content data of all accessions are listed in Supplementary Data 2. c Top schematic diagram depicts the classification of NMR19-4 into three different groups, i.e., methylated, unmethylated and deletion, and the leaf pictures below each group show darkness-induced leaf senescence phenotype in various A. thaliana accessions. Photographs were taken from the detached leaves before (0 d) or after incubation in darkness for 5 d, and pph-1, a null mutant of PPH in Col-0, was used as a control. Scale bars, 1 cm. d Quantification of chlorophyll content before and after 5 days of dark treatment in various accessions of A. thaliana. Chl, chlorophyll. Error bars are defined as s.e.m. (n = 3)
