Fig. 5

L230 promotes metastasis. a Scatter plot of numbers of lung metastases in each mouse (dots). Nude mice were injected intra-thoracically with H1299 cells that ectopically express full-length L230 or empty vector (Vec) to generate a single orthotopic lung tumor. After 4 weeks, mice were necropsied and visible tumors on the pleural surface of the contralateral lung were counted. b, c Migrated and invaded cells in Boyden chambers were imaged (b) and counted (c). H1299 cells that ectopically express full-length wild-type L230 (L230) or L230 containing mutations in an Fe²⁺-binding ligand (D827A, DA) or a 2-OG-binding ligand (R887A, RA) or empty vector (Vec) were seeded into the top chambers, and 10% fetal bovine serum was added to the bottom chambers as a chemoattractant. Scale bar = 400 µm. d Bar graph shows the average number of colonies formed by vector (Vec)- and L230-transfected H1299 cells in soft agar. e Relative densities of vector (Vec)- and L230-transfected H1299 cells in monolayer culture were determined at each time point by WST-1 assays. Results are mean values (±S.D.) from replicate biological samples. p values, two-tailed Student’s t test