Fig. 4

RNA-seq and ChIP-seq analysis shows that BMI1 regulates AR-signaling pathway independently of PRC1. a Heat maps for expression level of genes down- or upregulated by BMI1 or RING1B knockdown. b GSEA analysis showing enrichment level of AR-induced genes²⁸ in the genes downregulated by BMI1 or RING1B knockdown, and heat maps for expression level of the AR-induced genes. c Kaplan–Meier (KM) analysis of prostate cancer relapse based on expression level of 53 genes that were most significantly activated by BMI1 genes. TCGA gene expression data were collected from the Cbioportal database^49,50. Expression level of each gene in each sample was plotted in the heat map. d Venn diagram showing overlap of genes bound by AR and BMI1 as determined by ChIP-seq data. e Heat map showing the binding density of BMI1 and AR around individual binding sites of BMI1. f Number of genes bound by BMI1 and down- or upregulated by BMI1 knockdown. Number of genes expected by chance is also plotted. P value was calculated by Fisher’s exact test. g AR and IgG ChIP were conducted in C4-2 cells transfected with shBMI1 lentivirus or vector virus for 5 days. ChIP-qPCR was conducted using gene-specific primers