Fig. 2
From: Steric exclusion and protein conformation determine the localization of plasma membrane transporters
Substrate-dependent localization of proteins. Dual-color reconstructions of a Lyp1-L-YPet/Pil1-mKate2 and b Can1-L-YPet/Pil-mKate2 with and without lysine plus arginine in the growth medium, indicated as +KR and −KR, respectively. Wide-field images are depicted for clarity. All the scale bars represent 2 µm. c Cross-correlation of Pil1-mKate2 and Sur7-YPet. Panels: images were treated with a discoidal-averaging filter to better illustrate the localizations; the co-localization analysis was done with the raw diffraction-limited images. Wide-field images are depicted for clarity. d Number of localizations per cell of Lyp1 and Can1 with and without lysine plus arginine with error bars representing the standard deviation. e–h show cross-correlation of Pil1-mKate2 vs. proteins tagged with L-YPet; the left graph of each panel shows the correlation coefficients over distance for the various proteins with error bars representing standard error of the mean; the right graph of each panel shows the histograms of the probability distributions of single-cell cross-correlations. e Sur7 (blue; n = 118); f Lyp1 before addition of lysine plus arginine (green; n = 104), 40 min after the addition of lysine plus arginine (magenta; n = 138), and 120 min after the addition (blue; n = 108); g Can1 before addition of lysine plus arginine (red; n = 101), 40 min after the addition of lysine plus arginine (blue; n = 113) and 120 min after the addition (tan; n = 116); h Nha1 (light blue; n = 69). i, j Histograms showing the distance of Can1 molecules to the closest eisosome. Black lines indicate probability of finding an eisosomes at a discrete distance. i Can1 without arginine (n = 35); j Can1 with arginine (n = 47); n represents number of cells analyzed
