Fig. 4
Antiretroviral efficacy. a, b HIV-1 RT activity of (a) DTG and MDTG, and (b) NDTG and NMDTG-treated MDM. **P = 0.0039, ***P = 0.0007, ****P < 0.0001 MDTG vs. DTG and NMDTG vs. NDTG. c Prevention of spreading viral infection was assessed in human peripheral blood lymphocytes (PBLs) following addition of media conditioned from drug-treated MDM. NMDTG conditioned media significantly reduced HIV-1 RT activity in PBLs compared to NDTG conditioned media beginning at day 15 and maintained protection up to day 24. **P = 0.0018, ****P < 0.0001 NMDTG vs. NDTG. d Representative HIV-1p24 staining (brown) of virus-infected MDM-treated with native or nanoformulated drugs are shown. For all, uninfected cells without treatment served as negative controls. HIV-1-infected cells without treatment served as positive controls. Results were normalized to positive control cells. All results are shown as the mean ± SEM of three biological replicates. Results from a, b, c were analyzed by two-way ANOVA with Bonferroni’s multiple comparison tests
