Fig. 3

Functional interaction of UFD-2, UNC-45, and myosin in vivo. a Summary of crawling assays performed with unc-45(m94) and unc-45(m94) ufd-2(tm1380) adult worms at the restrictive temperature of 23 °C for 60 min. Representative images show tracks generated by a single worm. For quantification, movement behavior was classified into three different categories - unc (uncoordinated phenotype), mobile (reduced motility), and wild-type-like motility. b Western blot analysis showing UNC-45 protein levels in wild-type, unc-45(m94), and unc-45(m94) ufd-2(tm1380) adult worms. PGK-1 was used as a loading control. c Western blot analysis using an MHC-B specific antibody, showing levels of C. elegans muscle myosin in the indicated worm strains. d Quantification of the protein area of UNC-45 and UFD-2 in anti-UNC-45 and anti-UFD-2 IPs derived from parallel reaction monitoring (PRM). Two technical replicates are shown (two separate sets of IPs from the same lysate). No peptides were detected in control IPs