Fig. 5
From: UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins
Location of ubiquitination sites on the flexible UCS domain of UNC-45. a Upper panel: structural alignment of UNC-45815 (yellow) and UNC-45717 (lilac) revealed that ARM repeat 13 (red) serves as a molecular hinge connecting the two UCS subdomains and allowing the domain to adopt distinct overall folds (straightened UCS superhelix in UNC-45815, curved superhelix in UNC-45717). The structure on top is depicted in the same orientation as illustrations in Fig. 4. Lower panel: schematic model of UNC-45. Green spheres indicate KRcanyon mutations yielding a better UFD-2 substrate. b The subdomain architecture of the UCS domain is also seen when plotting the B factors on the molecular surface (green, rigid; magenta, flexible). c Ribbon presentation of the UCS domain of UNC-45815 (yellow) and UNC-45717 (blue), shown in similar orientation as in Fig. 4. The zoomed-in window shows the KRcanyon ubiquitination sites (green spheres) at the subdomain interface, as also indicated in a
