Fig. 6
From: UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins
KRcanyon mutations destabilize the UCS domain. a To visualize unfolded portions of the UNC-45 protein, limited proteolysis was carried out with trypsin. SDS-PAGE gels show trypsin digests of UNC-45 wild-type and mutant proteins pre-incubated for 60 min at 4 or 30 °C. Arrows highlight the disappearance of the 40 kDa fragment. b Degradation of the 40 kDa portion of UNC-45 illustrates the destabilizing effect of the UNC-45 KRcanyon mutations on the C-terminal of the UCS segment
