Fig. 4 | Nature Communications

Fig. 4

From: Disease-associated missense mutations in GluN2B subunit alter NMDA receptor ligand binding and ion channel properties

Fig. 4

Mg2+ permeability and block of NMDARs. a Ion permeability experiments using Mg2+ solution applied to HEK293 cells at −60 mV. Upper panel, NMDARs are exposed to 10 μM glutamate and 10 μM glycine either in nominally 0 Mg2+ Krebs (−Mg2+) or in Mg2+ solution (+Mg2+). Lower panel, bargraph of glutamate currents (IGlu) presented as a percentage IGlu in Mg2+ solution/IGlu in 0 Mg2+ Krebs. Membrane current in Mg2+ solution was only evident with GluN1–GluN2BV618G. One-way ANOVA with Dunnett’s post-hoc test, **p < 0.01. b I–V relationships in Krebs solution (with 1.2 mM Mg2+) generated by a voltage step protocol (from −90 to +90 mV) in the presence of 10 μM glutamate and 10 μM glycine. The curve fits used in all plots were generated using the trapping model (black: WT; red: N615I; blue: V618G)

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