Fig. 7

Ion channel mutants and Mg²⁺ block of NMDAR-mediated EPSCs. Hippocampal neurons (13–15 DIV) expressing GluN2B^WT (a, b), GluN2B^N615I (c, d) or GluN2B^V618G (e, f) subunits were recorded at −70 mV in Krebs with either 0 Mg²⁺ (Control, a, c, e) or + 1.2 mM Mg²⁺ (b, d, f). Bar graphs showing inhibition as a percentage of the mean peak EPSC amplitude (g), and charge transfer (area under the evoked EPSCs; (h) compared to controls in 0 Mg²⁺ for neurons expressing GluN2B^WT, GluN2B^N615I or GluN2B^V618G subunits at −70 mV. One-way ANOVA with Dunnett’s post-hoc test *p < 0.05, n = 5–7 cells for each condition. Calibration in a applies to b–f