Fig. 2
Light exposure affects C. elegans development. a Left, appearance of D1 adult N2 worms having been exposed to the four photoperiods from hatch; right, quantification of body surface from the left panel. b sod-3p::GFP worms exposed to permanent light or no light from hatch and observed at D1 adult under a GFP fluorescent filter (left) and quantification of the fluorescence (right); black rectangles indicate known areas of sod-3p::GFP expression where fluorescence was quantified. c daf-2(e1370) worms raised from hatch at 22 °C under permanent light or no light and quantified at +72 h for dauer or adult stage. daf-2(e1370);daf-16(mu86) strain was used as a negative control. d Lifespan of N2 worms raised until D1 adult under NL and from D1 onward under the four different photoperiods. In panels a and b, bars represent mean ± SEM, **P < 0.01; ***P < 0.001 using Student’s t test. #, P < 0.001 using Fisher’s exact test
