Fig. 5
Visible light creates photooxidation in C. elegans. a Relative expression levels of enzymes involved in oxidative stress response. b Microscope pictures of gst-4p::GFP worms exposed to PD or NL condition and observed under DIC lens (top panel) or GFP fluorescence (bottom). c Quantification of the GFP intensity from the experiment in b. d Oxyblot from worms exposed during 4 days to PD or NL. The top panel reveals DNP-derived proteins, as an indicator of oxidized proteins; β-actin antibody was used to verify equal loading (bottom panel). e Relative expression levels of mitochondrial UPR (UPRMT), endoplasmic reticulum UPR (UPRER), and cytosolic UPR (UPRCT) genes between worms exposed to PD or NL condition from hatch to D1 adult. f Microscope pictures of hsp-4p::GFP reporter worms under NL and PD conditions. Tunicamycin was used as positive control (Ct+) for induction of the ER-UPR40. g Quantification of the GFP intensity from the experiment in f. h Microscope pictures of hsp-6p::GFP reporter worms under NL and PD conditions. cco-1 RNAi was used as positive control (Ct+) for induction of the mito-UPR39. i Quantification of the GFP intensity from the experiment in h. j Microscope pictures of myo-3::GFPMT worms after 7 days of PD or NL condition and observed under DIC or GFP fluorescence with 63X objective; and k quantification of the overall mitochondrial integrity from the experiment in panel j. Bars represent mean ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001 using Student’s t test except for k: Fisher’s exact test
