Fig. 5

TRIM56^−/− mice are susceptible to HSV-1 infection but not to IAV infection. a HSV-1 (1.8 × 10⁸ pfu/mouse) was injected peritoneally to WT or TRIM56^−/− mice (n = 6 each). Survival rates were monitored for 7 days. b, c Sera were collected from WT or TRIM56^−/− mice injected with HSV-1 at the indicated time points, and IFNα or IFNβ concentration was measured by ELISA (n = 3 each at each time point). Total peritoneal cavity cells (d) and peritoneal macrophages (e) were isolated from WT or TRIM56^−/− HSV-1-infected mice (n = 3 for each time point). IFNβ mRNA levels in these cells were measured using RT-PCR. f Influenza PR8 (1000 pfu/mouse) was administered intranasally to WT or TRIM56^−/− mice (n = 9 each). Survival rates were monitored for 13 days. Error bars indicate mean ± s.d. *P < 0.05, Student’s t-test. Data in a–f are representative of two independent experiments. Error bars in b–e indicate mean ± s.d. of n = 3. *P < 0.05, versus control using Student’s t-test (a–e)