Fig. 6

TRIM56 is required for IFN induction in response to HSV-1 infection but not to IAV infection in primary cells. a RT-PCR analysis of IFNβ mRNA in BMDMs from WT and TRIM56^−/− mice at 18 h after mock, HSV-1, or HSV-1ΔICP34.5 infection. ELISA of IFNα (b) or IFNβ (c) in BMDMs from WT and TRIM56^−/− mice at the indicated time points after HSV-1ΔICP34.5 or HSV-1 infection. d WT or TRIM56^−/− BMDMs were infected with HSV-1 (MOI = 0.1). Viral supernatants were collected at the indicated time points, and virus titers were determined using a plaque assay on Vero cells. e RT-PCR analysis of IFNβ mRNA in WT or TRIM56^−/− BMDMs infected with lentivirus-derived vector, TRIM56 WT, or TRIM56 Mut. Results are presented relative to empty vector-expressing and mock-infected WT cells. f ELISA of IFNβ in MEFs from WT and TRIM56^−/− embryos. MEFs were stimulated with HT- DNA (2 μg/ml) or polydA:dT (1 μg/ml). g ELISA of IFNβ in BMDCs from WT and TRIM56^−/− mice at the indicated time points following infection with HSV-1ΔICP34.5. h ELISA of IFNβ in BMDMs from WT and TRIM56^−/− mice at the indicated time points following infection with IAV PR8. Data in a–g are representative of three independent experiments. Data in h are representative of two independent experiments. Error bars in a–h indicate mean ± s.d. of n = 3. *P < 0.05 versus control using Student’s t-test (a–h)