Fig. 3 | Nature Communications

Fig. 3

From: Cx26 drives self-renewal in triple-negative breast cancer via interaction with NANOG and focal adhesion kinase

Fig. 3

Localization of Cx26 in MDA-MB-231, MCF7, and MCF10A cells. a The cytosol and organelle fraction and the plasma membrane fraction of MDA-MB-231 parental cells were probed for a plasma membrane marker (sodium potassium ATPase), Cx26, and Cx43 by immunoblotting. b, c Cx26 localization in MCF7 and MCF10A cells was determined by immunoblotting of the plasma membrane and the cytosol and organelle fractions with Na/K ATPase as an indicator of plasma membrane fraction quality. d Immunohistochemistry micrographs demonstrate the staining pattern of Cx26 (brown) in normal and adjacent TNBC tissue from three patients. Nuclei were counterstained in blue. Images provided at 400×. e Confocal micrographs of MDA-MB-231 TNBC cells stained with antibodies against Cx26 (red) and lamin B1 (green). Single plane images and 3-D reconstructions of areas were generated in Imaris. Corresponding fluorescent images are provided above 3-D reconstructions. Nuclei were counterstained with Hoechst 33342, and scale bars are provided on each micrograph and reconstruction

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