Fig. 4 | Nature Communications

Fig. 4

From: Cx26 drives self-renewal in triple-negative breast cancer via interaction with NANOG and focal adhesion kinase

Fig. 4

Cx26 forms a TNBC-specific complex with focal adhesion kinase (FAK) and NANOG. Cell lysates from bulk cell cultures of a mesenchymal-like TNBC (MDA-MB-231), basal-like TNBC (HCC70), TNBC (PDX TN-1), and b non-TNBC (MCF7 and MCF10A) were subjected to immunoprecipitation with anti-Cx26, anti-FAK, and anti-NANOG antibodies. pFAK (Y397), FAK, Cx26, and NANOG proteins in the precipitated complex were detected by western blotting using specific antibodies. Fifteen percent of the lysate used for immunoprecipitation was loaded as the input control. As a negative control, immunoprecipitation with the corresponding non-immune IgG was performed. c Schematic summarizing the interactions detected in TNBC versus mammary epithelial and luminal breast cancer cells

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