Fig. 3
Genome-wide evaluation of modularity and self-sufficiency of TCSs in S. aureus. a Phenotypes of sequential TCS mutants: (i) nitrate reduction capacity, nitrite presence was colorimetrically detected; (ii) growth capacity on Triton X-100 concentration gradient agar plates; (iii) protein A expression in CDM detected by western blotting (full blots are shown in Supplementary Fig. 9); (iv) growth ability on TSA medium at 28 °C, serial dilutions were spotted on agar plates; (v) growth curves in TSB medium at pH 4.5, average and SD of three independent assays were recorded. TCSs whose mutation induces a phenotypic change are indicated. b Phenotypes of individual TCS mutants. Experimental conditions are described in a. c Phenotypes of selected individual and ΔXV mutant strains complemented with the corresponding TCS. Experimental conditions are described in a
