Fig. 1
From: 3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection
3D PHH cultures form physiological hepatic microtissues for extended periods of time. a Schematic of the perfused bioreactor, in which media is recirculated via a pneumatically driven micro-pump, and the collagen-coated scaffold for cell adherence. Each plate consists of 12 individual bioreactors, in which media flow can be regulated. b Cell viability of PHH following seeding in 3D scaffolds. Viable cells are stained green with calcein AM while dead cells are stained red with ethidium homodimer-1 3 days post-seeding. c Kinetic of hepatic microtissue formation and comparison to 3D spheroid, static 2D PHH, and SACC PHH hepatocyte morphologies. d Immunofluorescence microscopy of albumin (green) and DAPI (blue) in 2D PHH, 3D spheroid, SACC PHH, and 3D PHH cultures after 14 days of culture. e Secretion of albumin from 2D PHH, 3D spheroid, SACC PHH, and 3D PHH cultures after 14 days of culture as determined by ELISA. All data shown are mean ± SD of three to six independent experiments. p-values calculated by two-way ANOVA with Bonferroni post-test. Scale bars: white (200 μm), gray (500 μm)
