Fig. 6

Nestin⁺ cells at the injured site are predominantly MSPCs. a The percentage of the CD45^-GFP⁺ cells that express LepR. n = 16. b The percentages of the GFP⁺LepR⁺ cells or c GFP⁺LepR⁻ cells that express Sca-1, CD105, or CD31, respectively. d Alizarin red staining of GFP⁺ cells cultured in osteogenic differentiation medium for 14 days and e tube formation of GFP⁺ cells by Matrigel assay. f Immunostaining of COLII⁺ cells (red) and YFP⁺ cells (green) and g quantitation of Nestin-creERT2::EYFP^flox/flox mice 3 weeks post ATP. Scale bar, 50 μm, n = 8 per group. h, left: CD31⁺ cells (red), YFP⁺ cells (green), and right: Emcn⁺ cells (red), YFP⁺ cells (green), and i quantitation in ectopic bone marrow of Nestin-creERT2::EYFP^flox/flox mice 6 weeks post ATP. Scale bar, 100 μm, n = 8 per group. All data are shown as the mean ± s.d