Fig. 3

Functional inhibition of vWF causes prolonged bleeding in high shear conditions. a vWF (green) co-localizes with injured endothelial cells as well as with platelets. Endothelial cells are stained with plasma membrane stain (blue) while platelets are stained with anti-CD41 (red). b When healthy blood was treated with the AVW3 antibody, which binds and inhibits the A1 domain of vWF, we observed prolonged bleeding time only at arteriolar (2500 s⁻¹) shear rates comparted to venular shear rates (500 s⁻¹). One-way ANOVA indicate statistically significant difference among these four conditions, and Dunn’s multiple comparison test further show that bleeding times are significantly different between the 500 s⁻¹ control and 2500 s⁻¹ with AVW3 conditions (**). Scale bar = 50 µm. Bars in graph represent median values