Fig. 4 | Nature Communications

Fig. 4

From: Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection

Fig. 4

RXEG1 associates specifically with GH12 proteins in planta. a XEG1 associates with RXEG1, but not with RXEGL proteins or Arabidopsis RLP23 in planta. RLP23-GFP was used as a negative control. HA-tagged XEG1 was co-expressed with GFP-tagged RXEG1, the two RXEGL proteins, or RLP23 in N. benthamiana. Proteins were isolated at 2 dai, immunoprecipitated with GFP-trap® A beads, and subjected to western blotting with anti-GFP and anti-HA antibodies. b RXEG1 associates with different GH12 proteins in planta. NPP1 was used as a negative control. GFP-tagged RXEG1 was co-expressed with different GH12 proteins in N. benthamiana. Proteins were isolated at 2 dai, immunoprecipitated with GFP-trap® A beads, and subjected to western blotting with anti-GFP and anti-HA antibodies. c Assays for interaction between RXEG1 with XEG1 and various elicitors. GFP-tagged RXEG1 was co-expressed with HA-tagged XEG1, INF1, NPP1, Avh241, or EIX. Proteins were isolated at 2 dai, immunoprecipitated with GFP-trap® A beads, and subjected to western blotting with anti-GFP and anti-HA antibodies

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