Fig. 5 | Nature Communications

Fig. 5

From: Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection

Fig. 5

XEG1 associates with RXEG1 via the LRR domain in planta. a The LRR domain but not the SP + eJM + TM + tail (∆LRR) TM domain of RXEG1 binds to XEG1 in planta. HA-tagged XEG1 was co-expressed with a GFP-tagged LRR or ∆LRR domain of RXEG1, RXEGL1, or RLP23. Protein association was analyzed by co-immunoprecipitation (Co-IP) and western blotting. b The LRR domain of RXEG1 binds to XEG1 in the apoplast. XEG1 was co-expressed with GFP-tagged RXEG1SP+LRR, RXEGL1SP+LRR, or RLP23SP+LRR in N. benthamiana. Apoplastic fluid was collected 2 dai and subjected to Co-IP and western blotting

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