Fig. 3
From: CARM1-expressing ovarian cancer depends on the histone methyltransferase EZH2 activity
CARM1 promotes the silencing of EZH2 target genes and CARM1-regulated genes are enriched for SWI/SNF target genes. a Experimental strategy used to identify CARM1-regulated, clinically relevant EZH2/H3K27Me3 target genes. Ingenuity Pathway Analysis revealed that the top pathway enriched for the identified CARM1-regulated EZH2/H3K27Me3 target genes was apoptosis. Specifically, 19 of the 36 identified genes (including DAB2, DLC1, and NOXA) are known to promote apoptosis (see full list in Supplementary Table 2). b Examples of EZH2 and H3K27Me3 ChIP-seq and RNA-Seq tracks of the newly identified CARM1-regulated EZH2/H3K27Me3 target genes in the indicated parental control and CARM1 knockout A1847 cells. c Differentially expressed genes in control parental and CARM1 knockout A1847 cells identified by RNA-Seq (>3-fold) were subjected to Ingenuity pathway analysis for upstream regulators. The analysis revealed that SMARCA4 (also known as BRG1, a catalytic subunit of the SWI/SNF complex) was the top upstream regulator of these differentially expressed genes. d Expression of CARM1, BAF155, and BAF155Me in control parental and CARM1 knockout A1847 cells. Expression of β-actin was used as a loading control
