Fig. 5
From: CARM1-expressing ovarian cancer depends on the histone methyltransferase EZH2 activity
CARM1-mediated R1064 BAF155 methylation displaces BAF155 with EZH2 at the promoters of EZH2/BAF155 target genes. a CARM1-expressing A1847 cells were infected with a lentivirus encoding shBAF155 targeting the 3′ untranslated region (UTR) of the human BAF155 gene together with a retrovirus encoding wild-type BAF155 (WT) or a BAF155 R1064K mutant. Expression of BAF155 and BAF155Me was determined by immunoblot. β-actin expression was used as a loading control. b Same as a, but examined for the expression of the indicated BAF155/EZH2 target genes by qRT-PCR. *P < 0.001. c–e The indicated cells were subjected to ChIP analysis using anti-EZH2 (c), anti-H3K27Me3 (d), or anti-BAF155 (e) antibodies. An isotype-matched IgG was used as a negative control. ChIP products were subjected to qPCR analysis using primers specific for the promoter regions of the human DAB2, DLC1, and NOXA genes. Error bars represent SEM. P-values are from two-tailed t-test
