Fig. 4
Histone modifications of Fgf21 in Wy-offspring. a Schematic representation of the promoter region of Fgf21. Open circles and gray boxes indicate CpG sites and PPAR response elements (PPREs), respectively. BS, bisulfite sequencing. b, c ChIP assays of histone marks in Wy- and Veh-offspring at D16 (b) and 14W (c), with the indicated antibodies. Primers amplifying the region of −106 to +21 bp were used for ChIP-qPCR analysis (n = 5–6 per group). d ChIP assays of the recruitment of PPARα to PPRE1. Primers amplifying the region of −997 to −923 bp were used for ChIP-qPCR analysis (n = 4–8 per group). Statistics by unpaired Student’s t-test. Data are expressed as mean ± SEM. **P < 0.01; ***P < 0.001; N.S., not significant vs. Veh-offspring
